How to prepare phosphate buffer solution

How to prepare phosphate buffer solution

Phosphate buffer solution (PBS) is a buffer solution commonly used in biological laboratories and is widely used in cell culture, immunohistochemistry, molecular biology and other fields. This article will introduce the preparation method of phosphate buffer in detail, and attach a structured data table for readers' reference.

1. The role of phosphate buffer

The main function of phosphate buffer is to maintain a stable pH value of the solution and ensure the stability of experimental conditions. Its pH is usually 7.2-7.4, which is similar to the physiological environment and is suitable for most biological experiments.

2. Preparation method of phosphate buffer solution

The preparation of phosphate buffer requires the following reagents:

Preparation steps:

1. Weigh the above reagents, add 800 mL of distilled water, and stir until completely dissolved.

2. Use hydrochloric acid or sodium hydroxide to adjust pH to 7.2-7.4.

3. Dilute to 1 L, autoclave or filter sterilize before use.

3. Precautions for Phosphate Buffer

1. High-purity reagents must be used during preparation to avoid impurities affecting the experimental results.

2. When adjusting pH, operate slowly to avoid over-adjustment.

3. The sterilized buffer solution must be sealed and stored to avoid contamination.

4. Frequently Asked Questions about Phosphate Buffer

5. Summary

The preparation of phosphate buffer is a basic operation in biological experiments. Mastering the correct preparation method is crucial to the accuracy of experimental results. This article provides detailed recipes and steps, hoping to help readers.